Coding
Endolysin

Part:BBa_K5311006:Design

Designed by: Carlos Perez de Arenaza, Jose Luis Perez and Roger Planell   Group: iGEM24_UPF-Barcelona   (2024-09-24)


CAP 10-3 Endolysin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193


Design Notes

This sequence was codon optimized for Cutibacterium acnes


Source

You can find the endolysin [Cutibacterium phage CAP_10-3] using this coding in GenBank: WIW76974.1

References

[1] Kim JI, Hasnain MA, Moon GS. Expression of a recombinant endolysin from bacteriophage CAP 10-3 with lytic activity against Cutibacterium acnes. Sci Rep. 2023 Sep 30;13(1):16430. doi: 10.1038/s41598-023-43559-z. PMID: 37777575; PMCID: PMC10542754.